PCR experiment in order to ensure its relevance, accuracy, correct interpretation and repeatability. Assay carried out by pcr research paper lab or investigator’s lab? If frozen – how and how quickly?
If fixed – with what, how quickly? MIQE checklist for authors, reviewers and editors. If using primers obtained from RTPrimerDB, information on qPCR target, oligonucleotides, protocols and validation is available from that source. Assessing the absence of DNA using a no RT assay is essential when first extracting RNA. Once the sample has been validated as RDNA-free, inclusion of a no-RT control is desirable, but no longer essential. Disclosure of the probe sequence is highly desirable and strongly encouraged.
However, since not all commercial pre-designed assay vendors provide this information, it cannot be an essential requirement. Use of such assays is advised against. Jump to navigation Jump to search “PCR” redirects here. This article may be too technical for most readers to understand. Please help improve it to make it understandable to non-experts, without removing the technical details. The vast majority of PCR methods rely on thermal cycling.